microarray high-speed centrifuge arrayit mhc  (Arrayit Corporation)

Journal: BMC Microbiology

Article Title: Comparative transcription analysis and toxin production of two fluoroquinolone-resistant mutants of Clostridium perfringens

doi: 10.1186/1471-2180-13-50

Figure Lengend Snippet: Microarray and qRT-PCR analysis of the genes that were differentially affected in the gatifloxacin resistant mutants, NCTR R and 13124 R

Article Snippet: The hybridized arrays were washed twice in 6 × SSPE for 5 min at 60 ° C, once in 1 × SSPE for 5 min at 20 ° C, and once in 0.25 × SSPE at 20 ° C for 1 min, and then were spun dry in a microarray high-speed centrifuge (ArrayIT, model MHC).

Techniques: Microarray, Membrane, Planar Chromatography, Binding Assay

Journal: BMC Microbiology

Article Title: Comparative transcription analysis and toxin production of two fluoroquinolone-resistant mutants of Clostridium perfringens

doi: 10.1186/1471-2180-13-50

Figure Lengend Snippet: Microarray analysis of the genes that were upregulated in one or both gatifloxacin-resistant mutants, 13124 R and NCTR R

Article Snippet: The hybridized arrays were washed twice in 6 × SSPE for 5 min at 60 ° C, once in 1 × SSPE for 5 min at 20 ° C, and once in 0.25 × SSPE at 20 ° C for 1 min, and then were spun dry in a microarray high-speed centrifuge (ArrayIT, model MHC).

Techniques: Microarray, Membrane, Binding Assay

Journal: BMC Microbiology

Article Title: Comparative transcription analysis and toxin production of two fluoroquinolone-resistant mutants of Clostridium perfringens

doi: 10.1186/1471-2180-13-50

Figure Lengend Snippet: Microarray analysis of the genes that were downregulated in both gatifloxacin-resistant strains, 13124 R and NCTR R

Article Snippet: The hybridized arrays were washed twice in 6 × SSPE for 5 min at 60 ° C, once in 1 × SSPE for 5 min at 20 ° C, and once in 0.25 × SSPE at 20 ° C for 1 min, and then were spun dry in a microarray high-speed centrifuge (ArrayIT, model MHC).

Techniques: Microarray, Binding Assay